Figure 6

Typical images of the RPA-LFA (a–c) using rfbE F1/R1 primer set (targeting rfbE gene) and (d–f) stx2 F1/R1 (targeting stx gene) for the spiked commercial samples. From left to right, the concentrations of E. coli O157:H7 are 106 CFU/mL, 105 CFU/mL, 104 CFU/mL, 103 CFU/mL, 102 CFU/mL, 101 CFU/mL, 100 CFU/mL and 0 CFU/mL. (a, d) For drinking water samples, (b, e) for milk sample, (c, f) for apple juice sample.