Figure 4
From: Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
Phenotypic analysis of SlCHIP- or SlTPR28-silenced plants after heat stress. (A) SlCHIP and SlTPR28 transcript levels in SlCHIP- and SlTPR28-silenced tomato lines. The terminal leaflets in the fifth leaves of 20 individual 6-week-old plants were collected for RNA extraction and RT-qPCR analysis. Means of transcript levels were calculated from 12 plants with > 80% silencing efficiency and shown with SE (n = 12). These plants were used in the subsequent assays of heat tolerance. (B) Heat stress symptoms of SlCHIP- or SlTPR28-silenced plants. Six-weeks old mock (pTRV2), SlCHIP-silenced (pTRV2-SlCHIP) and SlTPR28-silenced (pTRV2-SlTPR28) plants were placed in a 45 °C growth chamber for 9 h. The picture was taken after 9 h heat treatment. (C) Water soaking symptom on leaflets of the fourth leaves in SlCHIP-silenced plants. Experiments were repeated three times with similar results.
