Figure 6
From: Functional analysis of tomato CHIP ubiquitin E3 ligase in heat tolerance
Accumulation of insoluble protein aggregates in SlCHIP- and SlTPR28-silenced plants under heat stress. Leaf tissues from mock (pTRV2)-, SlCHIP-silenced (pTRV2-SlCHIP) and SlTPR28-silenced (pTRV2-SlTPR28) plants were collected at indicated hours at 45 °C for extraction of total, soluble and insoluble proteins as described in Materials and Methods. The starting homogenates as total proteins and the last pellets as insoluble proteins were determined and the percentages of insoluble proteins to total proteins were calculated. Error bars indicate SE (n = 12). According to Tukey's multiple comparisons test (P = 0.01), means do not differ significantly if they are indicated with the same letter. Experiments were repeated three times with similar results.
