Figure 2 | Scientific Reports

Figure 2

From: Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel

Figure 2

Atelocollagen gel impacts the expression of MMP family members. (A) Sections prepared from three different samples on three time points. AC(+), AC(−), and atelocollagen sponge without cells were immunostained with anti-type I collagen antibody. Scale bar: 200 μm. (B) The concentration of hydroxyproline in the supernatant was determined on day 3 of culture, as described in the Materials and Methods. (C) RT-qPCR analysis of MMPs. Normalized expression relative to 2D culture cells is shown. (D) Representative images of immunostaining using anti-MMP9 antibody in AC(−) and AC(+) samples. Right panels are magnifications of the square regions in the left panels. (E) Comparison of the expression of factors related to chondrocyte differentiation. (C,E) A representation of three independent experiments, each with three subjects and normalized expression relative to 2D culture cells at approximately 50–60% confluency (semi-confluent monolayers) is shown. *p < 0.05, **p < 0.01.

Back to article page