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Figure 3

From: Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel

Figure 3

Gene expression in human articular cartilage-derived chondrocytes using a culture method. (A) Principal component analysis (PCA) of regularized-logarithm transformed read counts, in which three culture method groups were separated along the axis of major variation (PC1, 68% of variance). 2D: 2-dimensional culture, 3D: 3-dimensional culture. (B) Hierarchical clustering of 16,586 genes whose transcript levels changed by more than twofold or less than ½-fold, depending on the culture method. (C) Overlap of differentially expressed genes (DEGs) in the three comparisons: 2D culture versus 3D culture without atelocollagen gel, 2D culture versus 3D culture with atelocollagen gel, and 3D culture without atelocollagen gel versus 3D culture with atelocollagen gel. (D) Line graph representation of the transcript levels of genes shown in (B). The mapped data were analyzed using the Subio platform version 1.24 (Subio, Inc., Kagoshima, Japan, http://www.subio.jp).

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