Figure 4 | Scientific Reports

Figure 4

From: Integrin α2β1 plays an important role in the interaction between human articular cartilage-derived chondrocytes and atelocollagen gel

Figure 4

Atelocollagen gel significantly affects the expression of cell membrane- and extracellular matrix-related genes. (A,B) Gene ontology (GO) enrichment analysis comparing highly ranked (|log 2 FC|> 2) differentially expressed genes (DEGs) in 3D culture with atelocollagen gel and 3D culture without atelocollagen gel. (A) Enriched GO terms for the 1,863 genes upregulated in 3D culture with atelocollagen gel. (B) Enriched GO terms for the 2,056 genes down-regulated in 3D culture with atelocollagen gel. BP, biological process; CC, cellular component; MF, molecular function. (C) Heat map (logarithmic expression) of integrin family member genes presented in a mean enrichment plot across three culture groups. The mapped data were analyzed using the Subio platform version 1.24 (Subio, Inc., Kagoshima, Japan, http://www.subio.jp). (D) Comparison of expression of collagen-binding integrins. A representation of three independent experiments, each with three subjects and normalized expression relative to 2D culture cells at approximately 50–60% confluency (semi-confluent monolayers) is shown. *p < 0.05, **p < 0.01.

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