Figure 1
From: Direct and Indirect endocrine-mediated suppression of human endometrial CD8+T cell cytotoxicity
Effect of E2, P and TGFβ on endometrial CD8+T cells cytotoxicity. Purified endometrial CD8+T cells (effector cells, E) were pre-treated with E2 (5 × 10−8 M), P (1 × 10−7 M) for 48 h or TGFβ (10 ng/ml) for 2 h before co-culture with CFSE-stained allogeneic blood CD4+T cells (target cells, T) using a E:T ratio of 1:1. Cytotoxicity was measured over time by using quantitative time-lapse microscopy. Representative time-course of the kinetics of cytotoxicity over a period of 6 h in the absence (control) or presence of E2 (a), P (b) and TGFβ (c). Target alone are allogeneic blood CD4+T cells. Comparison of E2 (d, n = 7), P (e, n = 5) and TGFβ (f, n = 16) treatment in the mean number of dead target cells (left graph) and fold change in the mean number of dead target cells after E2, P and TGFβ treatment compared to control (right graph). Each dot represents a different patient. Min to Max (d–f, right graph) are shown. *P < 0.05; ** P < 0.01; Wilcoxon matched-pairs signed rank test.
