Figure 2
From: Direct and Indirect endocrine-mediated suppression of human endometrial CD8+T cell cytotoxicity
Effect of E2 and P on intracellular cytotoxic molecules in endometrial CD8+T cells. Mixed cell suspensions from endometrial tissues were pre-treated with E2 (5 × 10−8 M), P (1 × 10−7 M) for 48 h and stained for the intracellular cytotoxic molecules perforin, granzyme A and granzyme B for analysis by flow cytometry. (a) Representative histograms of intracellular perforin (left), granzyme A (middle) and granzyme B (right) expression in endometrial CD8+T cells treated with E2 and P. Gate boundaries (horizontal line) were set by fluorescence minus one (FMO) to indicate the gating of positive cells. (b) Median fluorescence intensity (MFI) of perforin (left), granzyme A (middle), granzyme B (right) and (c) percentage of perforin+ (left), granzyme A+ (middle), granzyme B+ (right) in endometrial CD8+T cells following incubation with E2 and P. Each dot represents a different patient (n = 5). *P < 0.05; Friedman test with Dunn’s multiple comparisons test.
