Figure 3
From: Direct and Indirect endocrine-mediated suppression of human endometrial CD8+T cell cytotoxicity
Effect of E2 on intracellular cytotoxic molecules in endometrial CD103+ and CD103−CD8+T cells. Mixed cell suspensions from endometrium were pre-treated with E2 (5 × 10–8 M) for 48 h and stained for the intracellular cytotoxic molecules perforin, granzyme A and granzyme B for analysis by flow cytometry. (a) Representative dot plots of intracellular perforin (left), granzyme A (middle) and granzyme B (right) expression in endometrial CD103+and CD103−CD8+T cells treated with E2. Top row shows control group (without E2) and bottom row shows E2 treated group. (b) Median fluorescence intensity (MFI) of perforin (left), granzyme A (middle), granzyme B (right) and (c) percentage of perforin+ (left), granzyme A+ (middle), granzyme B+ (right) in endometrial CD103+and CD103−CD8+T cells following incubation with E2. Each dot represents a different patient (n = 5). *P < 0.05; Friedman test with Dunn’s multiple comparisons test.
