Figure 5 | Scientific Reports

Figure 5

From: Remote liver ischemic preconditioning attenuates myocardial ischemia/reperfusion injury in streptozotocin-induced diabetic rats

Figure 5

Effect of RLIPC on morphological changes post-myocardial ischemia/reperfusion. (A) Representative (of n = 5 rats/group) H&E stained heart sections are shown. Panel (a) scale bars: 100 μm; panel (b) scale bars: 10 μm. Sham, sham surgery; CON, LAD ligation; RLIPC, remote liver ischemic preconditioning; DM: diabetic rats. (B) Morphological evaluation of myocardium damage in each heart (n = 5 mice per group). The severity of myocardial damage was graded from 0 to 3 (see the ‘Methods’ section). Data were presented as mean ± SEM; †††p < 0.001 compared with CON; and ‡p < 0.05 compared with DM-CON (by one-way ANOVA). (C) Representative TUNEL-stained heart sections. TUNEL-positive (green) cardiomyocytes were identified as apoptotic cells. Scale bars: 10 μm. (D) Bar graph showing the average percentage of TUNEL-positive nuclei in the ischemic regions (n = 5–6 rats per group). ††p < 0.01 compared with CON; and ‡‡‡p < 0.001 compared with DM-CON (by one-way ANOVA).

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