Figure 2
Preservation and impairment of the peripheral sensory nerves. Significant attenuation of the elevation of tail flick latency (A) was observed paralleling the preservation of CGRP-expressing DRGs (B) and in the spinal dorsal horn (C), at the 5th lumber segment of the animals from the capsaicin treated diabetic group, stained with antibodies for TRPV1 (in green) and CGRP (in red) and the mergers (in yellow), showing massive losses of the expressions of TRPV1 and CGRP in the spinal dorsal horn, especially in the superficial laminae (marked by the circle) at the end of the 8 weeks after induction of diabetes. The preservation of the DRG neurons co-expressing TRPV1 and CGRP (D), the medium diameter neurons (E) and the small diameter neurons (F). Control or Ctrl non-diabetic control group, Ctrl + Cap non-diabetic animals treated with capsaicin, Diabetic or Db diabetic group, Db + Cap diabetic animals treated with capsaicin, TON total observed neurons. *p < 0.05, compared to the control; **p < 0.01, compared to the control; ##p < 0.01, compared to the diabetic. The scales = 100 µm.
