Figure 1 | Scientific Reports

Figure 1

From: Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen

Figure 1

Invasion of a WT ASC monoculture spheroid into the surrounding collagen substrate, revealed via label-free OCM imaging and temporal speckle contrast. Traditional OCM imaging (top row) records scattering signals of cells and collagen alike. Temporal speckle contrast enables segmentation of volumetric data into synthetic ‘cell’ and ‘collagen’ channels (rows 2–5). Scale bars = 200 µm. See text for details. Time-lapse animation is available in Supplementary Video 1.

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