Figure 4
Visualization of 3D collagen displacements in the vicinity of an obese (ob/ob) ASC + MCF10AT1 co-culture spheroid at time t = 24 h. (a) Top-down view of the spheroid, using the same depth-to-color projection as in row 3 of Figs. 1 and 2. (b) Top-down rendering of the spheroid (shown in gray) accompanied by colored arrows which indicate displacement of the collagen matrix as measured with respect to its initial configuration at time t = 0 h. Arrow lengths have been exaggerated for visualization purposes. (c) Re-rendering of (b) from an isometric viewing angle. The flat surface of the spheroid corresponds to a region where the spheroid comes into contact with the coverslip bottom of the petri dish. Time-lapse animation is available in Supplementary Video 4.
