Figure 7

(a–c) Show UV fluorescence of RT-PCR products on 2% agarose gels stained with ethidium bromide. Bands for 500 and 200 bp on the DNA ladders are noted. The position of 219 bp is denoted in red. S-carp cohabitated sentinel carp, FHM fathead minnow, goldfish, B brain, G gill, K kidney, S skin, NTC no template control. In (b) “standpipe” indicates swabs of the tank outflow standpipe filer. CyHV-3 specific qPCR copy numbers/mL of tissue supernatant from corresponding tissues are displayed below each lane. Samples in (a) are representative tissues from S-carp from tank A (first 4 samples) and from tank C (last 4 samples). Samples in (c) are triplicate tests of FHM and goldfish tissues which had positive qPCR tests.