Figure 1 | Scientific Reports

Figure 1

From: Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats

Figure 1

Positional cloning of furue. (a) Physical map of the furue locus. The furue locus was defined by D12Rat67 and D12Tua1. Their physical positions are shown above the map (Rnor_6.0 assembly). The positions and directions of four characterized genes are indicated by horizontal arrows below the map. The deletion containing exon 6 (62 bp in length) of the Prkar1b gene of the furue genome is represented by a dashed line. Breakpoints of the deletion are indicated by arrowheads. Primers to detect the wild-type (a and b) and deletion (a and c) alleles are indicated by arrows. (b) Semi-quantitative RT-PCR analysis of Sun1 and Prkar1b mRNA. M, 50-bp DNA ladder molecular marker. (c) Genomic DNA PCR for genotyping the furue deletion. Primers a, b, and c represent rPrkar1b-wild-F, rPrkar1b-wild-R, and rPrkar1b-del-R, respectively. The positions of primers are depicted in Supplementary Fig. S2.

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