Figure 2
Development of Prkar1-deficient rats. (a) Target sequence of guide RNA in the CRISPR/Cas system. Exon 2 and intron 2 of the rat Prkar1b gene are represented in upper- and lowercase, respectively. The coding sequence is represented in bold. The target sequence of the guide RNA is underlined and the PAM sequence is highlighted in green. Primer sequences to detect mutations induced by the CRISPR/Cas system are indicated by horizontal arrows. (b) Genomic alternations induced by the CRISPR/Cas system. A 2-bp insertion was found in the target site of the Prkar1bem1 line. A 13-bp deletion was found in the target site of the Prkar1bem2 line. (c) Relative expression level of the Prkar1b transcript (left) and expression level of PRKAR1B protein (right) in the hippocampus of Prkar1b-deficient rats. Expression levels of Prkar1b transcripts are significantly decreased in mutant lines. PRKAR1B proteins are diminished in mutant lines. **P < 0.01.
