Table 1 Details on primers used for qRT-PCR analysis.
Gene | Gene description | Genbank ID | Primer sequences (forward primer/reverse primer, 5′–3′) | Product length (bp) | Amplification efficiency (%) | Correlation coefficient R2 |
|---|---|---|---|---|---|---|
UBQ | Polyubiquitin | KR349345 | 5′-TGCTTCGTCTCAGGGGTGG-3′ 5′-GTCCTGAATTTTAGCTTTCAC-3' | 116 | 102.5 | 0.991 |
TUB | Beta-tubulin | MN548043 | 5′-GTGCTGGTAATAACTGGG-3' 5′-GGGAAGACGGAGAAAGTA-3' | 226 | 97.6 | 0.992 |
EF-1α | Elongation factor-1α | MN548044 | 5′-TCAAGAAGGTCGGATACA-3' 5′-ACAGGGACAGTTCCAATAC-3' | 223 | 97.8 | 0.991 |
18S | 18S ribosomal RNA | KM656452 | 5′-CTGGTCTTTTCGGATGAT-3' 5′-CCTTTACGCCCAGTCATT-3' | 266 | 104.2 | 0.997 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | MK766385 | 5′-TTATCAACCCCACTACCA-3' 5′-TTCCTTCACCAAACACTC-3' | 203 | 98.6 | 0.995 |
ACT | Actin | MN548045 | 5′-GTCGCCCTCGCCATACAG-3' 5′-CTCTTCGGGAGCAACACG-3' | 194 | 101.8 | 0.988 |
TUA | Alpha-tubulin | MK778379 | 5′-ATTGGACAGGCTGGGATT-3' 5′-TTGGCAGCATCTTCTTTT-3' | 278 | 100.5 | 0.993 |
LcCu/Zn-SOD1 | Copper/zinc superoxide dismutase | KP178922 | 5′-CACAGGAAAGATGGTGAAGG-3′ 5′-CCAGCAGGGTTGAAATGT-3’ | 210 |
