Table 2 Expression stability of seven reference genes following various treatments based on a NormFinder analysis.

From: Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions

Rank

Heat

Cold

Salt

H2O2

Gene

SV

Gene

SV

Gene

SV

Gene

SV

1

EF-1α

0.595

EF-1α

0.243

UBQ

0.454

TUB

0.470

2

18S

0.716

TUB

0.418

TUA

0.822

EF-1α

0.801

3

ACT

0.858

18S

0.420

EF-1α

1.034

UBQ

0.906

4

TUA

0.860

GAPDH

0.451

TUB

1.365

ACT

0.909

5

UBQ

0.892

ACT

0.522

GAPDH

1.482

18S

1.542

6

TUB

1.127

UBQ

0.698

ACT

1.745

TUA

1.728

7

GAPDH

1.508

TUA

0.876

18S

1.811

GAPDH

1.862

Rank

ABA

Drought

All samples

Gene

SV

Gene

SV

Gene

SV

1

EF-1α

0.282

TUB

0.438

EF-1α

0.829

2

TUA

0.289

18S

0.726

TUB

0.877

3

TUB

0.500

UBQ

0.743

TUA

1.055

4

ACT

0.726

ACT

1.000

UBQ

1.121

5

18S

1.019

TUA

1.076

ACT

1.331

6

UBQ

1.451

EF-1α

1.145

18S

1.432

7

GAPDH

1.640

GAPDH

1.654

GAPDH

1.519

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