Table 3 Expression stability of seven reference genes following various treatments based on a BestKeeper analysis.

From: Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions

Rank

Heat

Cold

Salt

H2O2

Gene

SD

CV

Gene

SD

CV

Gene

SD

CV

Gene

SD

CV

1

ACT

0.149

0.59

ACT

0.298

1.16

UBQ

0.56

2.98

TUB

0.494

2.13

2

TUA

0.727

3.38

EF-1α

0.342

1.63

18S

0.883

4.31

EF-1α

0.513

2.7

3

TUB

0.746

3.08

18S

0.443

2.23

ACT

0.932

3.51

ACT

0.606

2.25

4

EF-1α

0.747

3.75

GAPDH

0.458

2.17

TUA

0.998

4.6

UBQ

0.777

3.99

5

18S

1.008

4.91

TUA

0.475

2.1

EF-1α

1.144

5.72

TUA

1.055

5.09

6

UBQ

1.325

6.66

TUB

0.495

2

GAPDH

1.288

6.23

18S

1.271

5.88

7

GAPDH

1.776

8.21

UBQ

0.657

3.24

TUB

1.534

6.71

GAPDH

1.288

6.39

Rank

ABA

Drought

All samples

Gene

SD

CV

Gene

SD

CV

Gene

SD

CV

1

TUA

0.44

1.9

TUB

0.553

2.25

ACT

0.875

3.33

2

ACT

0.484

1.87

TUA

0.554

2.46

EF-1α

0.957

4.71

3

EF-1α

0.617

2.9

EF-1α

0.794

3.84

TUA

0.958

4.34

4

18S

0.67

3.3

ACT

0.963

3.54

TUB

0.973

4.04

5

TUB

0.834

3.34

18S

0.966

4.63

18S

1.015

4.92

6

GAPDH

1.335

6.22

UBQ

1.062

5.25

UBQ

1.275

6.34

7

UBQ

1.604

7.27

GAPDH

1.34

5.87

GAPDH

1.443

6.77

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