Figure 1
From: In vitro optoacoustic flow cytometry with light scattering referencing
Schematic depiction and validation of the OA-FCM system. (a) The OA-FCM system is based on focusing a 532 nm laser into a microfluidic channel, which is connected to a syringe pump for controlling the flow of suspensions of particles or cells. OA and LS signals are recording simultaneously. (b) Close-up depiction of the microfluidic interrogation area. Raw (c) OA and (d) LS signal detected from a single bead indicated by an arrow flowing through the interrogation area flowing at 100 μL/min. (e) OA and (f) LS maximum amplitude projection of the record window shown in (c) and (d). (g) OA- and LS-trajectories recorded over 5 s show transits of individual beads. (h) Auto-correlation curves of the OA and LS trajectories in (e) reveal interrogation time of ~ 300 μs. Abbreviations: AE: active element; AcL: acoustic lens; AL: achromatic doublet lens; AMP: low noise amplifier; AWG: arbitrary waveform function generator; DAQ: data acquisition card; IM: inverted microscope; L: planoconvex lens; M: dielectric mirror; ND: neutral density filter; OL: microscope objective lens; PH: pinhole; S: high-precision motorized stage; SP: syringe pump; UT: Ultrasound transducer; W: Waste.
