Figure 2
From: Rhizobia use a pathogenic-like effector to hijack leguminous nodulation signalling

Phylogenetic and In silico analyses of B. elkanii Bel2-5 and its homologs. (a) Neighbor-joining tree of Bel2-5 and its homologs in T3SS-harbouring rhizobia and phytopathogenic bacteria. Boostrap values are given at the nodes with boostrap resampling (1000 replicates of each). (b) Schematic representation of Bel2-5 and XopD from X. campestris str. 85–10. (c) Schematic representation of Bel2-5 functional domains in B. elkanii USDA61, in comparison to its homologs in selected rhizobia and pathogenic bacteria. The repeat domain I and II, EAR motifs, NLS motif and ULP1 domain are shown. (d) Sequence alignment of ULP1 catalytic domain of B. elkanii BEL2-5 and its selected homologs, including: B. japonicum Is-34 (MA20_12780), B. diazoefficiens USDA110 (Bll8244), S. fredii HH103 (SFHH103_04358), Bradyrhizobium sp. XS1150 (NopD), M. loti MAFF 303,099 (Mlr6316), X. campestris pv. vesicatoria str. 85–10 (XopD), and yeast ULP1 (DAA11408.1). H, D and C catalytic core residues are indicated by red. Identical amino acids are shaded in black, and similar amino acids are shaded in gray, respectively.