Figure 1
Expression and distribution of S100A4 in differentiating and mature fibers of the mouse lens. (A) Immunofluorescent staining of S100A4 distribution (green) in sagittal sections of embryonic (E12.5, 13.5, 14.5 and 17.5) and differentiated (P21) mouse lenses. Nuclei are stained with Hoechst (blue). There seems to be no nuclear localization of S100A4 in lens fibers as shown in the bottom right panel (magnified image of the indicated area of E14.5 lens). LE lens epithelium, LV lens vesicle, LF lens fibers. Scale bars 20 μm. Representative images of one of three independent specimens analyzed are shown. (B) Immunoblot analysis of S100A4 in P21 mouse lens epithelium and fiber mass reveals its distribution specifically in fibers. (C) Immunoblot of S100A4 in P21 mouse lens lysates show its distribution primarily to the soluble (100,000 g supernatant) fractions compared to membrane fractions (100,000 g pellet). For soluble and membrane fractions, protein samples from the respective fractions separated on SDS-PAGE were stained with Gelcode blue with the staining intensity of the indicated protein band used as a loading control (LC). GAPDH was used as a loading control (B). Lanes 1 and 2 represent two biological replicates (B,C).
