Figure 3
From: Altered high-density lipoprotein composition and functions during severe COVID-19
Western blot analysis for detection of SAA and PON-1 in HDL isolated from COVID-19 patients and controls. HDLs (3 μg protein /lane) were immunoblotted for SAA (serum amyloid A) (A) and PON-1 (paraoxonase 1) (B). (A) HDLs isolated from patients and controls are presented individually (C1+ = ICU-COVID-19 positive patient #1, C2+ for patient #2, etc.) at day 1, 3 and 7 after admission (D1, 3 and 7). Controls 1 and 1′ correspond to HDLs isolated from healthy subjects sex- and age-matched to patient #1. (B) Pools of LDLs and HDLs were blotted for PON-1 (left panel) after isolation from non-infected healthy subjects ("Controls"), COVID-19 positive patients in intensive care unit ("C+ICU") (n = 8) and SARS-CoV-2 seropositive cured caregivers ("sero+", sampled > 15 days after symptoms, n = 7). A pool of control HDLs containing PON-1 was incubated with elastase (El, 20 μg/mL), plasmin (Pn, 500 μg/mL) or both proteases (El + Pn) for 2 h at 37 °C. Enzymes alone (without HDLs) treated in the same experimental conditions were loaded as control. Uncropped gels are presented in Supplementary information file.
