Figure 1
From: Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy
Cardiac-specific knockout of miR-125b-1 increased perinatal lethality and impaired systolic and diastolic heart function. (a) The design of the Cre-loxp system. The miR-125b-1 chromosome locus was flanked by loxp sequence. In the following definition, + / + indicates Myh6-cre; miR125b1 + / + and f/f indicates Myh6-cre; miR125b1f./f. (b) The expression of primary miR-125b-1 transcript by qPCR in the control and knockout neonatal ventricles (n = 3 in each group, p = 0.0142). (c) The expression of mature miR-125b by qPCR in the knockout neonatal ventricles (n = 3 in each group, p = 0.0062). (d) The expression of primary miR-125b-2 transcript by qPCR in the control and knockout neonatal ventricles (n = 3 in each group, p = 0.005). (e) The survival curve of cardiac-specific miR-125b-1 knockout mice (n = 27 vs. 64 in control and knockout mice). (f) The appearance of knockout neonates. (g) The body weight of control and knockout neonates (n = 34, 37 and 11 in the + / + control, f/f alive knockout and f/f dead knockout mice, one-way ANOVA with Tukey’s post-test was used. Adjust p < 0.001 in all comparisons). (h) The ventricular weight of control and knockout neonates (n = 18 vs. 17, p = 0.0388). (i) Echocardiography of neonatal hearts with M-mode view. (j) Ejection fraction in the neonatal left ventricles (n = 13 vs. 41, p = 0.0428). (k) Fraction shortening in the neonatal left ventricles (n = 13 vs. 41, p = 0.0633). (l) End-diastolic volume in the neonatal left ventricles (n = 13 vs. 41, p = 0.1353). (m) End-systolic volume in the neonatal left ventricles (n = 13 vs. 41, p = 0.0557). (n) Stroke volume (n = 13 vs. 41, p = 0.3642) and (o) cardiac output in the neonatal left ventricles (n = 13 vs. 41, p = 0.6975). (p) Left ventricular end-diastolic anterior wall thickness (n = 17 vs. 47, p = 0.3223). (q) Left ventricular end-diastolic posterior wall thickness (n = 17 vs. 47, p = 0.0038). (r) Left ventricular end-systolic anterior wall thickness (n = 17 vs. 47, p = 0.2640). (s) Left ventricular end-systolic posterior wall thickness (n = 17 vs. 47, p = 0.0415). (t) M-mode of mitral inflow by Doppler image in the neonatal left ventricles. (u) The velocity ratio of early filling (E wave) and late diastolic filling (A wave) in the neonatal hearts (n = 7 vs. 34, p = 0.0014). The significance of the differences between control and knockout groups were determined by unpaired Student t-test.
