Figure 3
From: Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy
MiR-125b deficiency promotes the progression of heart failure. (a) HE staining of young adult hearts. Bar: 2 mm. (b) Heart weight-to-body weight ratio of young adult mice (n = 19 vs. 14, p = 0.0044). (c) Ejection fraction (n = 19 vs.. 14, p = 0.0262), (d) end-diastolic volume (n = 19 vs.. 14, p = 0.1211) and (e) end-systolic volume (n = 19 vs.. 14, p = 0.4270) in young adult mice. (f) The ratio of early diastolic mitral inflow velocity to early diastolic septal mitral annulus velocity (E/E' ratio) (n = 19 vs. 14, p = 0.06049) and (g) the ratio of early diastolic mitral inflow velocity to early diastolic lateral mitral annulus velocity (E/E' ratio) (n = 19 vs. 14, p = 0.3833) in young adult mice. (h) HE staining of mature adult mice. Bar: 2 mm. (i) Heart weight-to-body weight ratio of mature adult mice (n = 10 vs. 18, p = 0.0358). (j) Ejection fraction (n = 10 vs. 18, p = 0.0077), (k) end-diastolic volume (n = 10 vs. 18, p = 0.1489) and (l) end-systolic volume (n = 10 vs. 18, p = 0.0894) in mature adult mice. (m) The ratio of early diastolic mitral inflow velocity to early diastolic septal mitral annulus velocity (E/E' ratio) (n = 6 vs. 6, p = 0.0132) and (n) the ratio of early diastolic mitral inflow velocity to early diastolic lateral mitral annulus velocity (E/E' ratio) (n = 6 vs. 6, p = 0.0353) in mature adult mice. (o) Quantification of cardiomyocyte size by WGA staining in the 11-week-old adult myocardium (n = 9 vs. 7, p < 0.0001). (p) βMHC, ANP, BNP and Acta1 gene expression in the young adult myocardium (n = 8 vs. 6, βMHC p = 0.5999; ANP p = 0.0395, BNP p = 0.0587, Acta1 p = 0.0069). (q) Quantification of cardiomyocyte size by WGA staining in the 6-month-old adult myocardium (n = 6 vs. 7, p = 0.0063). (r) TEM of the 6-month-old adult myocardium. Bar: 2 µm. (s) TEM of the 6-month-old adult myocardium with electron-dense mitochondrial contact sites, indicated by red arrows. Bar: 0.1 µm. (t) Numbers of droplets under 10,000 × field by TEM (n = 4 vs. 6, p = 0.0306). (u) Percentage of droplet area in tissues under 10,000 × field by TEM (n = 4 vs. 6, p = 0.0015). The significance of the differences between control and knockout groups were determined by unpaired Student t-test.
