Figure 4
From: Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy
Mitochondrial miRNA-125b expression in neonatal and adult hearts. (a) Confirmation of isolated mitochondria from neonatal hearts. Vdac2 and Cox4 are mitochondrial proteins and αtubulin is a cytosolic protein. MT: mitochondria. Vdac2, Cox2 and αtubulin were cropped from the same blot. The original images of blot were showed in Fig. S7. (b) Mitochondrial miRNA-125b expression in neonatal hearts. A spike-in control, cel-mir-39, was used to normalize the technical error (n = 3 vs. 4, p = 0.0015). (c) MiRNA-125b expression in young adult myocardium (n = 4 vs. 3, p = 0.9667). (d) Pri-miR-125b-1expression in young adult myocardium (n = 7 vs. 5, p = 0.0139). (e) Pri-miR-125b-1expression in young adult myocardium (n = 7 vs. 5, p = 0.5758). (f) Pri-miR-125b-1 expression in isolated cardiomyocytes from young adult myocardium (n = 4 vs. 3, p = 0.0053). (h) Pri-miR-125b-2 expression in isolated cardiomyocytes from young adult myocardium (n = 4 vs. 3, p = 0.8961). (i) Cytosolic miR-125b expression in young adult hearts (n = 6 vs. 5, p = 0.1874). (j) The ratio of mitochondrial miR-125b to cytosolic miR-125b in young adult hearts (n = 6 vs. 5, p = 0.0470). The significance of the differences between control and knockout groups were determined by unpaired Student t-test.
