Figure 6
From: Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy
Knockout of Tp53 rescued the increase of heart rates and end systolic pressure in cardiac miR-125b deficient mice, but not cardiac hypertrophy phenotype. (a) Expression of HIF1a and p53 ser15phosphorylation in wild-type and miR-125b knockout neonatal hearts. The original images are displayed in Fig. S5. HIF1a, p53 ser15phosphorylation and Gapdh were from different blots with the same protein extracts. (b) HE staining of hearts in the control (125b + / + ; Tp53 + / +), miR-125b knockout (125bf/f; Tp53 + / +) and miR-125b/Tp53 double knockout (125bf/f; Tp53−/−) mice. Bar: 1 mm. (c) Heart weight-to-body weight ratio in the control, miR-125b knockout and miR-125b/Tp53 double knockout mice (n = 5, 6 and 4. control vs. miR-125b knockout, p = 0.0450; control vs. double knockout, p = 0.1146; miR-125b knockout vs. double knockout, p = 0.9549). (d) Cardiomyocyte size in the control, miR-125b knockout and miR-125b/Tp53 double knockout mice (n = 6, 7 and 4. control vs. miR-125b knockout, p = 0.0112; control vs. double knockout, p = 0.2536; miR-125b knockout vs. double knockout, p = 0.4138). (e) The heart rate (control vs. miR-125b knockout, p = 0.0116; control vs. double knockout, p = 0.8295; miR-125b knockout vs. double knockout, p = 0.1760), (f) end-systolic pressure (control vs. miR-125b knockout, p = 0.0363; control vs. double knockout, p = 0.3478; miR-125b knockout vs. double knockout, p = 0.0055), (g) dP/dt maximum (control vs. miR-125b knockout, p = 0.0396; control vs. double knockout, p = 0.7684; miR-125b knockout vs. double knockout, p = 0.0265 ), (h) dP/dt minimum (control vs.vs. miR-125b knockout, p = 0.0028; control vs. double knockout, p = 0.8520; miR-125b knockout vs. double knockout, p = 0.0058), (i) Tau (control vs. miR-125b knockout, p = 0.2908; control vs. double knockout, p = 0.9843; miR-125b knockout vs. double knockout, p = 0.5825), (j) the slope of end-systolic pressure volume relationship (control vs. miR-125b knockout, p = 0.6666; control vs. double knockout, p = 0.5919; miR-125b knockout vs. double knockout, p = 0.9349) and (k) the slope of end-diastolic pressure volume relationship (control vs. miR-125b knockout, p = 0.7139; control vs. double knockout, p = 0.8718; miR-125b knockout vs. double knockout, p = 0.5113) in control, miR-125b knockout and miR-125b/Tp53 double knockout mice (n = 9, 12 and 4). The significance of the differences among control, knockout and double knockout groups were determined by one-way ANOVA with Tukey’s multiple comparison test.
