Figure 2
From: The role of gene to gene interaction in the breast’s genomic signature of pregnancy
Genetic signature of pregnancy through gene-by-gene analysis. Heatmaps. (A) Heatmap of all 2123 probes found differentially expressed (adjusted p value < 0.05) between nulliparous (left column) and parous (right column). Two heatmaps are presented to show specific features of the FFTP genomic signature associated with up/down regulation. Each horizontal line corresponds to the same probe in both heatmaps. The left heatmap clusterizes according to similarity of expression levels (red to green: low to high expression level). The right heatmap shows downregulation (red) and upregulation (green). As the histograms (blue curves) depict, upregulation for parous is more frequent in the central orange/yellow region (low/intermediate expression values in nulliparous). For high expression values (bottom region), downregulation for parous is slightly more frequent than overregulation. There is a general tendency to upregulation in parous, also found by 27, with an up/down regulation ratio of 2.17. (B) Heatmap of changes in entropy for the 18,022 probes showing significant changes (p value < 0.05). Cluster analysis shows a generalized gain in entropy induced by FFTP, which is not related to the entropy level in nulliparous (histograms/blue curves): The redefinition of the channels and stimuli reaching genes and their performance of additional and more complex functions as a consequence of FFTP homogenously affects all genes.
