Figure 2 | Scientific Reports

Figure 2

From: In vivo silencing of amphiregulin by a novel effective Self-Assembled-Micelle inhibitory RNA ameliorates renal fibrosis via inhibition of EGFR signals

Figure 2

Establishment of AD-induced mice and kinetic analysis of AREG expression, fibrosis-related genes, and inflammatory genes in the kidneys. (a) Time scheme for AD-induced mice verification. (b) Representative images of kidneys from control or AD-induced mice (harvested seven days after AD). (c) Histological analysis by H&E, MT, and IHC staining of control and AD mice. Tubular dilatation, the fibrotic areas, and the α-SMA-positive areas were quantified. Scale bar, 100 μm. (d) Kinetics of the expression of AREG, fibronectin, TGF-β1, α1(I) collagen, and α1(III) collagen in the kidneys of AD mice. (e) The kinetics of the expression of inflammatory genes (TNF-α and MCP-1) and molecular adhesion genes (VCAM-1 and ICAM-1) in AD-induced kidneys were measured using real-time qRT-PCR. The values in each panel represent the mean ± SEM of six mice induced by AD. **p < 0.01, ***p < 0.001 compared to control mice.

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