Figure 2 | Scientific Reports

Figure 2

From: A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis

Figure 2

Super-resolution live-and-dead fluorescence imaging of P. gingivalis incubated with buffer only (control) and SCPPPQ1 (20 µM). Both qualitative visual evaluation (insets) and quantitative evaluation of the fluorescence (also see Fig. S3), show that there are no significant changes in fluorescence intensity throughout the incubation interval for the control, while there is a major drop when cells are incubated with SCPPPQ1. Quantification of the bacteria (upper right panel) shows that between the 0 and 120 min interval the number of bacteria almost doubles whereas with SCPPPQ1 it remains static. In all cases, very few dead cells (red) are evident. Green = Syto9 labelling; Red = Propidium iodide. Data represent the means ± SD (n = 3).

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