Figure 4
SFN induce cell apoptosis in GC cells. (A) Increased apoptosis induction by SFN in BGC-823 and MGC-803 cells by Hoechst staining (× 200), the arrow indicates the apoptotic cells. The apoptosis rate in BGC-823 (B) and MGC-803 (C) cells treated with different concentration of SFN for 48 h was detected by flow cytometry after Annexin V/PI double staining. The apoptosis rate was calculated and depicted in a bar chart, and the values indicate the mean ± SD for three separate experiments, the statistical values were determined by Student’s t-test, *P < 0.05, **P < 0.01.
