Figure 5
The effect of SFN on the expression of cell cycle and apoptosis related proteins in a dose-dependent manner in GC cells. (A) The S phase arrest associated proteins p53, p21 and CDK2 associated proteins were examined by western blot analysis after treated with the increased concentration of SFN for 48 h in BGC-823 and MGC-803 cells. (B) Western blotting was used to analyze the effect of SFN on the expression of apoptosis-related proteins caspase-3 and Bax in BGC-823 and MGC-803 cells. (C) Inhibition of p53 activity caused by pifithrin-α reduces the expression of p21 and Bax in GC cells. (*P < 0.05, **P < 0.01).
