Figure 2
From: Lipid tethering of breast tumor cells reduces cell aggregation during mammosphere formation
PEM + lipid coated wells generate mammospheres with greater efficiency and smaller size compared to low attachment wells: for (A,C) FACS was used to seed single cell suspensions of MCF7 cells at densities of either 10, 100 or 1000 cells into low attach and PEM + lipid coated well plates. For (B,D) T47D cells were employed. (A,B) At low seeding densities of 10 cells/well or 100 cells/well, the average size of mammospheres formed is comparable in low attach and PEM + lipid wells. At a seeding density of 1000 cells/well, there are significantly different averages of spheres in the low attach and PEM + lipid wells, respectively. (C) The total number of mammospheres formed in low attach and PEM + lipid coated wells have no significant difference when 10 MCF7 cells/well or 100 MCF7 cells/well are seeded. However, when 1000 cells/well were seeded, there were significantly different averages between the low attach and PEM + lipid coated wells. (D) The total number of mammospheres formed in PEM + lipid coated wells have no significant difference when 10 T47D cells/well are seeded. However, when 100 or 1000 cells/well were seeded, there were significantly different averages between the low attach and PEM + lipid coated wells. (×40 magnification, bar 50 μm). Statistics formulated using ANOVA with post-hoc Tukey HSD. *p value < .01.
