Figure 3
From: Lipid tethering of breast tumor cells reduces cell aggregation during mammosphere formation
Lipid tethering reduces the effects of aggregation: single cell suspensions of MCF7 and T47D cells were used to seed 1000 cells into low attach and PEM + lipid coated well plates and were analyzed on Day 7 post-seeding. (A) Low attachment wells have no significant difference in distribution of mammospheres in the well center and at the well edge for either MCF7 or T47D cells, whereas lipid coated wells have a significant difference of > 20% between spheres in the center and edges of the well in both cell lines. (B) Panel (B) is a visual representation of sphere size distribution in the wells. Edge size distribution at the edge is displayed in the outer ring. Well center distribution is displayed in the inner circle. Values are in percentages. (B, upper) Low attach plates have a nearly equal size distribution in the center of the well, with the edge having a 75% (± SD6) population of spheres over 150 μm. PEM + lipid wells have only 36% (± SD4) spheres over 150 μm at the well edge and 24% (± SD3) in the center. (B, lower) Representative images of T47Ds greater propensity to form aggregations, low attach plates have higher concentrations of spheres > 80 μm in both the well center and well edge. PEM + lipid plates however have 93% and 81% clonal spheres in the center and at the edge, respectively. (Statistics formulated using ANOVA with post-hoc Tukey HSD. *p value < .01, **p < .001) C) Representative images of wells seeded with 1000, T47D cells in either low attach or PEM + lipid plates and imaged on day 7. Red circle marks 500 μm from the well edge.
