Figure 4
From: Lipid tethering of breast tumor cells reduces cell aggregation during mammosphere formation
Lipid-coated wells have a higher proportion of clonal mammospheres compared to low attachment wells: (A) FACS was used to seed single cell suspensions of MCF7-GFP and MCF7-mCherry cells into 96 well plates. 500 cells of each cell type were sorted together into each well of low attachment and lipid-coated plates. Confocal imaging of mammospheres on Day 7 post-seeding shows that mammospheres in the low attachment plates are positive for both GFP and mCherry expression (top panels, white arrows), whereas the majority of the mammospheres observed in the lipid-coated plates are positive for either GFP expression or mCherry expression (lower panels, white arrows)—×10 magnification, scale bar 200 μm. (B) Quantification of mammospheres greater than 60 μm indicates that low attachment wells have significantly more double-colored mammospheres whereas PEM + lipid wells have significantly less. Statistics formulated using ANOVA with post-hoc Tukey HSD. *p value < .01.
