Figure 2
AM- and plasma-derived HIV primary isolates are T-tropic. HIV env isolated from AMs or plasma from 3 HIV-infected donors was cloned into an HIV NL4-3 env deleted backbone plasmid. Replication-competent virus was added to activated CD4+ T cells or MDMs for 12 h and washed, and supernatant was collected 1 h after washing (Day 1) and after 7 or 14 days. Data is shown as fold change of HIV p24 in supernatant at the final timepoint (Day 7 for T cells, Day 14 for MDMs) compared to Day 1. Cells were infected with AM-derived viral isolates (A) from viral donor 1, 2 or 3 or plasma-derived viral isolates (B) from viral donor 1, 2 or 3. Data from all viral isolates from the same viral donor and anatomical site were pooled. CD4 + T cells and MDMs were isolated from six HIV-uninfected blood donors. In (A), n = 12 (6 cell donors infected with 2 viral isolates) for viral donor 1, n = 18 (6 cell donors infected with 3 viral isolates) for viral donor 2, and n = 18 (6 cell donors infected with 3 viral isolates) for viral donor 3; in (B), n = 12 (6 cell donors infected with 2 viral isolates) for viral donor 1, n = 12 (6 cell donors infected with 2 viral isolates) for viral donor 2, and n = 30 (6 cell donors infected with 5 viral isolates) for viral donor 3. Statistics by Kruskal–Wallis test. *p < 0.05, **p < 0.01, ***p < 0.001.
