Figure 5
From: HIV induces airway basal progenitor cells to adopt an inflammatory phenotype
HIV-treated BC release GM-CSF that stimulates AM proliferation. (A) Quantification of GM-CSF in BC-conditioned medium by ELISA. (B) Proliferation of human AM is partially mediated by BC-released GM-CSF in conditioned media. Alveolar macrophages in serum-free RPMI were plated in 96-well and incubated with BC-conditioned media from untreated, HIV-treated and heat-inactivated HIV-treated BC culture. RPMI and recombinant GM-CSF (20 ng/ml) serve as background and positive controls, respectively. BC-conditioned media were pre-treated with mouse IgG1 control and anti-GM-CSF neutralizing antibody (both at 10 µg/ml) for 30 min prior to addition to the cells. Cell proliferation was measured by BrdU incorporation assay after 3 days of culture. Data represent the mean ± SD of one representative experiment (n = 3) performed in triplicate.
