Figure 3 | Scientific Reports

Figure 3

From: Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems

Figure 3

The preparation of the semitransparent amniotic membrane (stAM) as the matrix for cell culture, and the effects of the stAM on inverted microscopy imaging quality impairments. (a–c) The harvest of the stAM and the preparation as the matrix for cell culture in a transwell. (d,e) The spatial relationship of the stAM, bottom of the transwell, cultivated cells, objective lens of the FF-OCT, and light emitted for obtaining images. (f,g) The stAM was semitransparent and reduced light transmission, and both transwells were added to the culture medium. (h,i) The stAM reduced the imaging quality of cultivated cells under a phase-contrast inverted light microscope.

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