Figure 4 | Scientific Reports

Figure 4

From: Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems

Figure 4

The effects of the stAM on the imaging quality taken by FF-OCT. (a–c) Neuron cells (N2A) cultivated on the stAM. (a) Hematoxylin and eosin (H&E) staining of cross-sectional neuron cells cultivated on the stAM. Yellow arrow: neuron cells. Blue star: stAM. (b) A 2D cross-sectional FF-OCT image of neuron cells cultivated on stAM. Yellow arrow: neuron cells. Blue star: stAM. (c) A 3D FF-OCT image of neuron cells cultivated on stAM. Yellow arrow: cell body of the neuron cell. Red arrows: neuron processes. Blue star: stAM. (d–k) The effects of the stAM on the FF-OCT cross-sectional and en face imaging quality of cultivated mCLES. (d,e) H&E staining of the cross-sectional mCLESs with or without stAM as the matrix. Blue star: stAM. (f,g) A 2D cross-sectional FF-OCT images of mCLESs with or without stAM as the matrix. Blue star: stAM. (h–k) Sequential FF-OCT en face images at 5 μm (h,i) and 30 μm (j,k) above the bottom of the basal epithelial cells. Scale bar = 20 μm. mCLESs: multilayered cultivated limbal epithelial sheet.

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