Figure 6 | Scientific Reports

Figure 6

From: Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems

Figure 6

The histological, 3D, and 2D cross-sectional FF-OCT images of multilayered cultivated cell sheets. (a) FF-OCT 3D images of DACNs. Yellow arrows indicate the outline surface of DACNs. Red arrows indicate the epithelial type of cells spread at the bottom of DACNs. (b) FF-OCT 2D images of DACNs. Yellow arrows indicate the outline surface of DACNs. (c,d) Hematoxylin and eosin (H&E) staining of cross-sectional DACNs revealed two different cell types, with epithelial cells covering the entire superficial surface of the colonies of neuron-like cells. (e) A 2D cross-sectional FF-OCT images of DACNs demonstrated epithelial-like cells covering the surface of neuron-like cells. (d,e) Green arrows indicated superficial epithelial cells. Red arrows indicated neuron-like cells. (a,b) Scale bar = 100 μm. (c) scale bar = 50 μm. (d,e) scale bar = 25 μm. DACN: directly adherent colonies of neuron-like cells.

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