Figure 3 | Scientific Reports

Figure 3

From: Oxycodone self-administration activates the mitogen-activated protein kinase/ mitogen- and stress-activated protein kinase (MAPK-MSK) signaling pathway in the rat dorsal striatum

Figure 3

Increased MSK1 and MSK2 protein phosphorylation in LgA-H rats. (A,F) Images of western blot and (B,C,G,H) quantification of MSK1, pMSK1, MSK2, pMSK2. (B) MSK1 protein levels are decreased in LgA-L and LgA-H rats. (C) pMSK1 protein abundance is upregulated in LgA-L and LgA-H rats. (D) pMSK1/MSK1 ratios are increased in LgA rats; (E) Changes in pMSK1/MSK1 ratios are dependent on oxycodone intake. (G) MSK2 protein levels were not significantly affected by oxycodone. (H) pMSK2 abundance is increased in LgA-H rats. (I) pMSK2/MSK2 ratios are increased in LgA-H rats and (J) correlated with amount of oxycodone. (n = 5–6 Sal; n = 6 ShA; n = 6 LgA-L; n = 6 LgA-H). Full-length blots are presented in Supplementary Figure S5. Key to statistics: *, **, *** = p < 0.05, 0.01, 0.001, respectively, in comparison to Sal rats; #, ##, ### = p < 0.05, 0.01, 0.001, respectively, in comparison to SHA rats; $$ = p < 0.01 in comparison to LgA-L rats. Statistical analyses are as described in Fig. 2.

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