Figure 4
Liver triglyceride (a), liver patatin-like phospholipase domain-containing protein 3 (lPNPLA3) mRNA expression (b), lPNPLA3 protein abundance (c), and adipose PNPLA3 (aPNPLA3; d) protein abundance during the experimental period for control (CTL) and ketosis induction protocol (KIP) treatments (TRT). The − 28 expected days relative to calving (DRTC) values were included as model covariates for their respective responses (b–d); values graphically represented for − 28 expected DRTC are the arithmetic means. Variance in the actual DRTC sampled is represented for the relevant prepartum timepoints (± standard deviation). Error bars represent the 68% confidence limits of the least squares or arithmetic mean. Symbols represent significant (*P ≤ 0.05) and marginal (†0.05 < P ≤ 0.10) simple effect differences for treatment × time interactions after multiplicity correction (Bonferroni). Differences across expected days relative to calving (DRTC; P ≤ 0.05, Tukey's adjustment) are indicated when alphabetic superscripts do not share characters.
