Figure 1

SAL prevents S. aureus biofilm dispersion. (a) Biofilm formation by the Newman strain or Newman agr mutant exposed to 2 mM of SAL measured after 24 and 48 h of static incubation at 37 °C. Each bar represents the mean ± SEM of 8 wells from 4 independent experiments. Biofilm formation values correspond to the OD₅₉₅ of crystal violet (OD_B) measured relative to the final culture density (OD_G) after 24 or 48 h incubation. Comparison of Newman treated with SAL and Newman agr treated with SAL at both time points showed no significant differences. Statistical analysis was performed with the Mann–Whitney test. (b) Scanning electron microscopy (SEM) representative images of Newman and Newman agr biofilms grown statically for 24 h at 37 °C in the presence or absence of 2 mM of SAL. The black bars correspond to 10 μm. The magnification is 2000×.