Table 2 Characterisation of nanobody binding affinity and head/stem specificity.

From: Nanobodies mapped to cross-reactive and divergent epitopes on A(H7N9) influenza hemagglutinin using yeast display

Nanobody

Affinity (KD)

A/Brisbane/10/2007 (H3N2) HA02

A/Chick/Netherlands/2003 (H7N7) HA02

A/Shanghai/01/

2013

(H7N9)

HA02

A/HongKong/125/

2017

(H7N9)

HA11

NB7-01

-

 < 0.1 nM

4.80 nM

0.83 nM

NB7-02

-

6.19 nM

17.3 nM

6.24 nM

NB7-03

-

24.80 nM

35.70 nM

27.45 nM

NB7-04

-

8.74 nM

26.9 nM

10.79 nM

NB7-05

-

140 nM

6.96 nM

 > 1 µM

NB7-07

-

0.81 nM

4.31 nM

0.78 nM

NB7-08

-

1.26 nM

2.22 nM

15.48 nM

NB7-09

-

 < 0.1 nM

0.38 nM

0.93 nM

NB7-10

-

24.0 nM

21.6 nM

46.24 nM

NB7-11

-

4.60 nM

58.8 nM

18.74 nM

NB7-12

-

1.05 nM

1.50 nM

3.72 nM

NB7-13

-

> 1 µm

–

> 1 µm

NB7-14

-

< 0.1 nM

2.36 nM

0.41 nM

NB7-15

-

0.42 nM

1.24 nM

0.77 nM

NB7-16

-

1.63 nM

9.98 nM

4.95 nM

NB7-17

-

1.43 nM

1.30 nM

2.40 nM

NB37X-01

#

-

#

-

NB37X-02

#

-

#

-

NB37X-03

#

-

#

-

NB37X-04

#

-

#

-

NB37X-05

#

-

#

-

NB37X-06

#

-

#

-

  1. 1 HA1 is purified hemagglutinin head domain.
  2. 2 HA0 is purified full length hemagglutinin.
  3. # binding seen but could not be fitted using 1:1 fitting model.
  4. - no binding could be seen.
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