Figure 7
Immunohistochemical changes after 20 min of S1-tDCS. (A,B) Confocal photomicrographs (images), quantification and statistics (bar charts) of GAD 65–67 (A) or vGLUT1 immunoreactivity (A) in S1 after 20 min of cathodal tDCS (upper row, n = 4 mice, two-way mixed ANOVA, F2,10 = 5.163, ***p < 0.001), after 20 min of anodal tDCS (middle row, n = 5 mice) and after 20 min of sham condition (lower row, n = 4 mice). (C) Same analysis but for adjacent motor cortex. Error bars represent SEM. GAD 65–67: Glutamic acid decarboxylase isoforms 65 and 67; vGLUT1: vesicular glutamate transporter 1; OD: optical density; IR: immunoreactivity; A.U.: arbitrary units.
