Figure 1

Purification of NS-CotA. (a) Coomassie-blue stained, PAGE analysis of extracts from non-induced (1), or IPTG-induced (2) E. coli transformed with dual expression plasmid muNS-Mi-CotA-pDuet1. Extracts from E. coli transformed with plasmid muNS-Mi-pDuet1 and induced with IPTG are shown in 3. (b) Protein content of the purified NS-CotA produced under standard (1) or microaerobic (2) conditions, analyzed by SDS-PAGE and Coomassie staining.