Figure 2
From: A recombinase polymerase amplification assay for rapid detection of rabies virus
In silico analysis of (A) the Schlottau assay and (B) the RABV-RPA assay again sequences of differences rabies virus isolates from Laos (LAO), China (CHIN), Brazil (BRE), Niger (NIG), Afghanistan (AFG) and Senegal (SEN), respectively. The alignment was performed using the MAFFT algorithm implemented in the Unipro UGENE software50. The in silico analysis revealed signature erosion in the reverse primer of the Schlottau assay, indicating that this rabies virus diagnostic method have issues with sensitivity when used to detect some isolates from these countries.
