Table 4 Oligonucleotide sequences of primers and probe designed for the RABV-RPA assay.

From: A recombinase polymerase amplification assay for rapid detection of rabies virus

Name

Type

Length (bp)

Sense

Sequence 5′–3′

Gene

Positiona

Product size (bp)

RPA_N_FP2C

Primer

35

Forward

GCATCCTTAGTCGGTCTGCTCTTGAGTCTGTATAG

N

482–516

133

Exo_probe_N

Probe

45

Reverse

CTATCCGATCTGCAA[BHQ-dT][THF][FAM-dT]TTGTCTTGTAGTTGCCTGTGTTTTGTCCTG–Ph

N

531–578

RPA_N_RP4C3P

Primer

32

Reverse

CAATTTTAACGAAAGGGGCTGTCTCAAAAATC

N

583–614

  1. FAM: fluorescein amidit; BHQ: blackberry quencher; THF: tetrahydrofuran; Ph: phosphate; bp: base-pairs.
  2. aCorresponding nucleotide positions on genome of the rabies virus strain from Nigeria DRV-NG11(GenBank Ac. No. KC196743).
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