Figure 2 | Scientific Reports

Figure 2

From: Reaction mechanism of the farnesyl pyrophosphate C-methyltransferase towards the biosynthesis of pre-sodorifen pyrophosphate by Serratia plymuthica 4Rx13

Figure 2

LC–MS separation of SAM, FPP, and putative PSPP in MRM-mode. Mass transitions are given in the method section. Enzyme assays including FPP and SAM were incubated, (A) with FPP C-methyltransferase enzyme (FPPMT), (B) without FPPMT. FPP and PSPP (red) elute at very similar retention times. Since no PSPP formation was observed in the control (B), the formation of PSPP (A) is enzyme-dependent. The split peak for SAM is due to detector overload.

Back to article page