Figure 1
From: MDM4 inhibition: a novel therapeutic strategy to reactivate p53 in hepatoblastoma
MDM4 expression is significantly elevated in HB patient samples and is correlated with increased expression of p53 target genes. (a) Dot plot representing expression profiles of MDM4 and MDM2 and normalized averages of the mRNA expression profiles of 22 select p53 target genes (AEN, ALDH4A1, CDKN1A, DDB2, DUSP1, EDA2R, ESR1, FAS, FDXR, GADD45A, GADD45B, PANK1, PERP, PTCHD4, RPRM, RPS27L, RRM2B, SPATA18, TRIAP1, TRIM22, WRAP53, ZMAT3), together with the optimal linear fit. (b) Dot plot representing expression of MDM4 and MDM2, together with the optimal linear fit. (c) Bar graph representing normalized mRNA expression of MDM2 and MDM4 analyzed with qPCR experiments with 18 patient samples in comparison to four adjacent uninvolved liver samples. Y-axis shown with a log2 scale. Error bars represent SD. Data shown in b are representative of at least three independent experiments performed with three replicate wells each time. Student’s t test (two tailed) *P < 0.05, **P < 0.01, ***P < 0.001 representing comparison of each sample’s expression data to all expression data of uninvolved liver samples.
